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Drug Resistant Bacteria Takedown Underway


Validation of biofilm formation on human skin wound models and demonstration of clinically translatable bacteria-specific volatile signatures

The purpose of this experiment was to develop and assess bacterial biofilm formation and identify their unique VOC profiles to help stop the creation of antibiotic resistant bacteria at an early stage. It was also to supply experimental models to assess theranostics (combining specific targeted therapy based on specific targeted diagnostic tests). The scientists grew biofilms on plastic coverslips for one, three, and five days, at 37 degrees Celsius in incisional and excisional human cutaneous wound tissues in a specific broth medium. They used six different methods to evaluate the formations and their structure was reviewed. The Volatile organic compounds (VOCs), which are unique to various disease states and can be associated with these certain biofilms, were identified using gas chromatography. All their experiments were done twice in triplicate for accuracy in their results.
Some flaws were that they had only used three bacterial strains and that the ex vivo study did not have the ability to have completely controlled conditions and it could’ve impacted the VOCs. The conclusions of the experiment were that they identified 12 specific peaks associated with biofilm growth and development based on the present VOCs and successfully cultured three pathogenic bacterial biofilms and paved the way for potential clinical applicability of diagnosing of cutaneous wound infections. The findings will allow other scientists to use their experimental models and findings to understand delayed wound healing and stop antibiotic resistant bacteria before an infection is allowed to heal.

Comments

  1. AnonymousSeptember 7, 2018 at 11:20 AM

    1. Do they expect to be able to stop MRSA in any way soon? Or will it be much later in time?


    2. What types of VOCs or bacterias exist in these biofilms?

    ReplyDelete
  2. AnonymousSeptember 7, 2018 at 11:21 AM

    1. What types of bacteria did they use?
    2. What other compounds did they find with the GC?

    ReplyDelete
  3. AnonymousSeptember 7, 2018 at 11:21 AM

    1. What bacterial species were found in the biofilms?
    2. What is the current antibiotic used to treat biofilms? Does one exist yet?

    ReplyDelete
  4. MaricarSeptember 7, 2018 at 11:21 AM

    1. Are there medical centers known for their theranostics programs? Which schools?
    2. What conditions were controlled in the experiment?

    ReplyDelete
  5. AnonymousSeptember 7, 2018 at 11:21 AM

    1. How recent was this study?
    2. Are there any other fields this research and findings can be applicable to?

    ReplyDelete
  6. AnonymousSeptember 7, 2018 at 11:21 AM

    1. Which other bacteria have these new antibiotics responded to?

    2. Which VOCs specifically made the staff bacteria antibiotics the most successful?

    ReplyDelete
  7. AnonymousSeptember 7, 2018 at 11:22 AM

    1. What types of compounds did the GC find in the biofilms?

    2. How will scientists use the GC to improve antibiotic resistance?

    ReplyDelete
  8. AnonymousSeptember 7, 2018 at 11:22 AM

    1. Do we know if the results could apply to other bacterial strains besides the ones studied?
    2. How can they isolate the specific reasons behind the causes of the peaks if there are so many bacteria?

    ReplyDelete
  9. AnonymousSeptember 12, 2018 at 11:42 AM

    1. Are there medical centers known for their theranostics program? Which schools?

    I did not find a complete list of schools with the top theranostics program, but the one school that had multiple studies including Theranostics was the University of Tennessee Graduate School of Medicine. They had multiple studies published about their theranostics program.
    http://gsm.utmck.edu/research/ACTP/main.cfm

    2. How recent was this study?

    This study was published in 2018. This study is extremely recent, but it may have took one to two years for the work to be reviewed and published into an article.
    https://www.nature.com/articles/s41598-018-27504-z

    3. What types of compounds did the GC find in the biofilms?

    The GC found twelve peaks which indicated 3-methylbutanal, pentanal, hydrogen cyanide, 5-methyl-2-hexanamine, 5-methyl-2-heptamine, 2-nonanone, 2-undecanone, 2-methyl-1-propanol, 3-methyl-1-butanol, 2-methyl-1-propanol, and 3-methyl-1-butanol.
    https://www.nature.com/articles/s41598-018-27504-z

    4. Are there any other fields this research can be applicable to?

    This research can be applied to cancer treatment. The University of Tennessee Graduate School of Medicine is using to combat cancer. This research could be the some of the first blocks towards fighting cancer through theranostics.
    http://gsm.utmck.edu/research/ACTP/main.cfm

    ReplyDelete

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